The authentication method of the habitat of insect

The authentication method of the habitat of insect

  • CN 101,712,991 B
  • Filed: 09/25/2009
  • Issued: 03/16/2016
  • Est. Priority Date: 09/26/2008
  • Status: Active Grant
First Claim
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1. a method, is characterized in that, it is the making method of the mark of habitat for the identification of the described insect belonged in the insect of red flour beetle and lesser grain borer, and the method comprises following operation:

  • A () determines the operation of the DNA base sequence of the insect of more than a kind from the habitat of more than 2;

    B operation that () is compared to the base sequence determined in above-mentioned operation (a);

    (c) according to the comparison result of above-mentioned operation (b), by the operation that the site of the base of more than 1 conservative in the whole above-mentioned base sequence of supply comparison formation is removed from above-mentioned base sequence;

    D (), according to the removal result in above-mentioned operation (c), will remain all or part of operation as type identification site in site;

    E () is for the type identification site obtained in above-mentioned operation (d), relatively mutually corresponding base, on all four type identification site is categorized as same type, not quite identical type identification site is categorized as the classification operation of the type of other more than 1;

    WithF (), for all kinds of classification in above-mentioned operation (e), determines all types of habitats by belonging to all types of insect habitats, thus using the operation of all types of type identification sites as mark,Further, the DNA base sequence of above-mentioned insect is more than a kind that selects from the base sequence from mitochondrial ND5 gene and COI gene,Further, foregoing tags is the base of the 22nd that 5 '"'"' end of the base sequence from red flour beetle COI gene shown in sequence number 1 starts is A, the base of the 142nd is A, the base of the 229th is G, the base of the 253rd is T, the base of the 298th is for any one in G or more than 2 and/or be the base of the 248th that starts of 5 '"'"' end of the base sequence from red flour beetle ND5 gene shown in sequence number 8 for A and/or be the base of the 160th that 5 '"'"' end of the base sequence from lesser grain borer COI gene shown in sequence number 34 starts be T.

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