Preparation method of trinary composite stent of plasmid DNA / fibrin gel / polymer

Preparation method of trinary composite stent of plasmid DNA / fibrin gel / polymer

  • CN 101,716,382 A
  • Filed: 12/02/2009
  • Published: 06/02/2010
  • Est. Priority Date: 12/02/2009
  • Status: Active Grant
First Claim
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1. the preparation method of plasmid DNA/fibrin gel/polymer ternary compound rest, its step is as follows:

  • 1) quaternised chitosan is dissolved in the phosphate buffer, is mixed with the n-trimethyl chitosan chloride solution of 1-10mg/mL, through 0.22 μ

    m membrane filtration degerming;

    Plasmid DNA is made into the aqueous solution of 100-1000 μ

    g/mL;

    The plasmid DNA aqueous solution is mixed with n-trimethyl chitosan chloride solution equal-volume, and the concuss mix homogeneously leaves standstill, and prepares the compound particle of plasmid DNA;

    2) with fibrinogenolysis in normal saline, be mixed with the fibrinogen solution that concentration is 10-80mg/mL;

    Thrombin is dissolved in the calcium chloride solution, and being mixed with concentration is the thrombin calcium chloride solution of 10-20U/mL;

    3) porous polymer scaffold is immersed in the ethanol, adopts first evacuation, the method for pressure is recovered in the back, and ethanol is imported in the porous polymer scaffold;

    To import alcoholic acid support and be immersed in No. three distilled water, and carry out first evacuation repeatedly, the method for pressure is recovered in the back, and ethanol is replaced as water, and is residual until no ethanol;

    4) utilize negative pressure to be filled in the porous polymer scaffold simultaneously the compound particle of isopyknic plasmid DNA, fibrinogen solution and thrombin calcium chloride solution, to prop up to be placed in the constant temperature oven and hatch, make Fibrinogen solidify the formation hydrogel fully, promptly obtain plasmid DNA/fibrin gel/polymer ternary compound rest.

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