Method for batch production, separation and purification of catechin monomers EGCG

Method for batch production, separation and purification of catechin monomers EGCG

  • CN 101,723,927 A
  • Filed: 12/02/2009
  • Published: 06/09/2010
  • Est. Priority Date: 12/02/2009
  • Status: Active Application
First Claim
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1. a method for batch production, separation and purification of catechin monomers EGCG is characterized in that:

  • may further comprise the stepsA, single-column extracting and separating;

    with rough tea-polyphenol solution and eluent upper prop, the high 15m of post, diameter 55cm, make eluent with 75% ethanol, control tea-polyphenol solution flow rate 700-900L/min, ethanol flow velocity 1500-1700L/min extracted time 1.5-2 hour, reclaim the elutriant that contains L-L-Epicatechin gallate catechin monomers EGCG with reduced vacuum, again chromatography is treated in its storage;

    B, low pressure column chromatography purifying;

    with KR100-5C18 is column packing, the high 2.8m of post, with 25% aqueous ethanolic solution is eluent, elution flow rate 450L/min, carry out column chromatography to reclaiming the concentrated back elutriant that contains L-L-Epicatechin gallate catechin monomers EGCG to be purified, collect catechin monomers EGCG target liquid by time or volume, to catechin monomers EGCG target liquid reclaim concentrated, crystallization, lyophilize gets catechin monomers EGCG finished product.

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