Method for batch production, separation and purification of catechin monomers EGCG

Method for batch production, separation and purification of catechin monomers EGCG

  • CN 101,723,927 B
  • Filed: 12/02/2009
  • Issued: 05/13/2015
  • Est. Priority Date: 12/02/2009
  • Status: Active Grant
First Claim
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1. a method for batch production, separation and purification of catechin monomers EGCG, is characterized in that:

  • comprise the following steps;

    (1) first drop into 1200kg tealeaves, to heat immersion tealeaves with deionized water, soak three times;

    (2) suction filtration tea;

    (3) respectively twice suction filtration mixed solution is concentrated;

    (4) upper chromatography column;

    1. the pre-treatment of filler;

    take KR100-5C18 300kg, fully swelling with eluent 25% ethanol 400kg;

    2. slowly pour swelling good filler into chromatography column, make its uniform settling, in time being deposited to 2.75m, close feeding port, with transferpump by drip washing in 25% ethanol input post of 5-8 times of column volume, to balance pillar;

    3. upper prop;

    about 200L extracts polyphenol medicinal extract solution, with in transferpump input post, infiltrate completely after in post until sample, connect collector again, input 25% ethanol with low-pressure delivery pump to start to collect, first paragraph is EGC, is followed successively by D, L-C, CAF, EGCG, EC, GCG, ECG, elution flow rate is 450L/min, collects 800L altogether;

    4. adopt high performance liquid chromatograph, respectively qualitative analysis is carried out to the composition and content of collecting liquid;

    (5) merge the elutriant of the EGCG catechin monomers containing ECG, concentrate 40 minutes at 100L vacuum concentration unit;

    1. fill post;

    slowly poured in post by swelling good filler, make its uniform settling, in time being deposited to 2.75m, closing and fill out post mouth valve, with transferpump conveying 5-8 50% ethanol rinse doubly, to balance pillar;

    2. upper prop;

    collect liquid by concentrating the catechin monomers of rear EGCG and ECG solid content about 50, with in transferpump input post, infiltrate completely after in post until sample, adopt senior liquid phase chromatography, qualitative analysis is carried out to composition, the content collecting liquid, merges the elutriant containing EGCG catechin monomers, in 50L concentrating under reduced pressure unit, after being concentrated into the solid content containing 45%, be further purified crystallization, lyophilize obtains finished product.

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