Method for improving salt-tolerant properties of Brassicaceae crops

Method for improving salt-tolerant properties of Brassicaceae crops

  • CN 101,731,137 A
  • Filed: 11/13/2008
  • Published: 06/16/2010
  • Est. Priority Date: 11/13/2008
  • Status: Active Grant
First Claim
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1. method that improves the crop in cruciferae salt-tolerance character, this method comprises:

  • 1) after the seed sterilization with this crop, flooded 10-40 hour, take out the back aseptic water washing with the reagent that is selected from bleomycin A5, ethylmethane sulfonate, Sodium azide, dithyl sulfate, nitroso ethyl carbamide, 5-bromouracil and maleic acid hydrazide or its combination;

    2) step

         1) gained seed is inoculated on the 1/2MS medium that contains NaCl0.6-1.6% (w/v), makes its germination, extract true leaf out;

    3) bud with above-mentioned extraction true leaf downcuts, and is inoculated in the 1/2MS medium, treats that it grows to 3-6 sheet true leaf, selects normal bud seedling and stays;

    4) the normal bud seedling of the band 3-6 sheet true leaf that step

         3) is obtained changes the 1/2MS medium that contains NaCl0.6-1.6% (w/v) over to, and after 20-40 days, the bud seedling of choosing survival changes the 1/2MS medium over to and recovers to cultivate;

    5) terminal bud of the band 3-6 sheet leaf of the plant that will cultivate through over recovery downcuts, change 1/2MS medium culture 20-40 days of NaCl0.6-1.6% (w/v) over to, screening stays the bud seedling that the leaf look dark green, stem is healthy and strong, change over to the 1/2MS medium that adds the 0.1-10mg/L basic element of cell division by strain system recover, enrichment culture;

    6) will downcut through the terminal bud of the band 3-6 sheet leaf of the salt-resistance strain of over recovery, enrichment culture, change in the 1/2MS medium of the auximone that adds NaCl0.6-1.6% (w/v), the 0.1-10mg/L basic element of cell division, 0.01-5.0mg/L and carry out culture of rootage, after 20-40 days, observe the upgrowth situation of each strain system, add up the frequency of taking root of each strain system, growing way stalwartness, rooting rate are preserved in the system of the strain more than 70%;

    With7) strain of step

         6) gained being tied up to the 1/2MS medium that adds the 0.1-10mg/L basic element of cell division breeds, expands numerous, with 20-30 days be the one-period successive transfer culture once, every 2-4 all after date, change in the 1/2MS medium that contains NaCl0.6-1.6% (w/v) and carry out selection of salt tolerance, obtain salt-resistance strain.

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