cell sorting system and method

cell sorting system and method

  • CN 101,743,303 B
  • Filed: 04/11/2008
  • Issued: 12/14/2016
  • Est. Priority Date: 04/20/2007
  • Status: Active Grant
First Claim
Patent Images

1. Microfluidic cell sorter, comprising:

  • It is suitable for accepting the cell import of the one or more cells in fluid media (medium);

    It is fluidly connected to the first and second buffer agent imports of cell import, to provide buffer agent solution to sorter;

    It is fluidly connected to cell import and the fluid passage of the first and second buffer agent imports,Being fluidly connected to the first cross-flow passes of fluid passage, described first cross-flow passes is positioned at the first and second fluid cushion agentThe downstream of import;

    Being fluidly connected to the second cross-flow passes of fluid passage, described second cross-flow passes is positioned at the first and second fluid cushion agentThe downstream of import, and the second cross-flow passes be positioned at fluid passage with on the first cross-flow passes opposite side;

    Being fluidly connected to the first and second outlets of fluid passage, these outlets are positioned at the downstream of the first and second cross-flow passes,The cell being suitable for detection given state the cell responding given state produce the detector of signal, and this detector is arrangedCell is detected for the upstream position at the first cross-flow passes,Be connected to detector and the first and second cross-flow passes pneumatic cross force switch, its response signal and actuatable with producePneumatic pressure pulses, thus cause fluid to move in cross-flow passes, described pneumatic cross force switch includes;

    It is connected to the first pneumatic operated valve of the first cross-flow passes;

    It is connected to the second pneumatic operated valve of the second cross-flow passes;

    Connect detector and the control system of pneumatic cross force switch, it is provided that timing controling signal actuates the first He in a sequential mannerSecond pneumatic operated valve, it is characterised in that the front opening that the first pneumatic operated valve is opened at the second pneumatic operated valve;

    Thus when the cell of given state being detected, pneumatic cross force switch is activated to provide cross force to cell, thusMake signaling so that its selectivity exits into the first or second outlet.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×