Under the situation that does not have ristomycin, measure the method for von willebrand factor activity and the method for mensuration ADAMTS-13 proteinase

Under the situation that does not have ristomycin, measure the method for von willebrand factor activity and the method for mensuration ADAMTS-13 proteinase

  • CN 101,755,211 A
  • Filed: 07/03/2008
  • Published: 06/23/2010
  • Est. Priority Date: 07/06/2007
  • Status: Active Application
First Claim
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1. in sample, measure the active method of vWF ELISA (VWF) for one kind, wherein, with described sample with separate GPIb α

  • albumen and be mixed into a kind of detection sample, it is characterized in that,A) use the separation GPIb α

    albumen that comprises a kind of amino acid sequence, described amino acid sequence is compared with the wild-type sequence (SEQ ID NO;

    1) of human GPIb α

    albumen and is comprised amino acid residue 1-268 at least, and on position 233 and 239, have a kind of aminoacid replacement base Xaa respectively, andB) in described detection sample, do not add ristomycin and America spearhead abdomen toxalbumin.

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