The method for disulfide formation of improvement

The method for disulfide formation of improvement

  • CN 101,772,574 B
  • Filed: 08/21/2008
  • Issued: 01/06/2016
  • Est. Priority Date: 08/21/2007
  • Status: Active Grant
First Claim
Patent Images

1. for by the first cysteine residues in the coat protein being contained in phage particle with form disulfide linkage between the second cysteine residues being contained in peptide/protein and by the method for described peptide/protein exhibiting expression on the surface of phage particle, described method comprises:

  • A (), in adjacent described first cysteine residues be contained in the described coat protein of phage particle or 5 amino acid being contained in described second cysteine residues in described peptide/protein, Methionin is introduced in artificially,WithB () causes or makes the described coat protein of phage particle be connected to described peptide/protein,Wherein said connection is by described first cysteine residues in the described coat protein being contained in phage particle and forms disulfide linkage between described second cysteine residues being contained in described peptide/protein and cause,And wherein said peptide/protein comprises immunoglobulin (Ig) or its function fragment.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×