Method for quickly detecting six kinds of genetically modified corns

Method for quickly detecting six kinds of genetically modified corns

  • CN 101,812,527 B
  • Filed: 04/20/2010
  • Issued: 09/17/2014
  • Est. Priority Date: 04/20/2010
  • Status: Active Grant
First Claim
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1. a method for 6 kinds of transgenic corns of rapid detection, is characterized in that following steps:

  • Step 1, optical thin film biologic sensor chip are prepared;

    Described optical thin film biologic sensor chip is silicon material optical thin film biologic sensor chip, and chip is divided into three layers, is followed successively by from top to bottom silicon supporting layer, reflection layer, surface attachment layer;

    The specificity of transformant sequence selection of step 2, transgenic corns, probe and design of primers;

    Exogenous gene sequence on 6 kinds of transgenic corns kind DNA sequence dnas and the DNA sequence dna on position, and in the gene order comprising, at connecting zone, choose the DNA fragmentation of 100~

    300bp length, make this fragment both comprise the exogenous gene sequence that part proceeds to, comprise again the peculiar gene order of this corn variety of part, obtained the specificity of transformant sequence of this kind of transgenic corns kind;

    In described 6, transgenic corns kind is respectively Bt11, Event176, GA21, MON810, NK603, T25;

    Described probe and the design of primer are as follows;

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