Method for separating and purifying desertliving cistanche polysaccharide

Method for separating and purifying desertliving cistanche polysaccharide

  • CN 101,870,742 B
  • Filed: 06/30/2010
  • Issued: 07/02/2014
  • Est. Priority Date: 06/30/2010
  • Status: Active Grant
First Claim
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1. a separating and purifying method for Herba Cistanches polysaccharide, is characterized in that the concrete steps of described method are:

  • (1) extract;

    Herba Cistanches is cleaned and dries rear pulverizing, respectively by the sherwood oil of 2-5 times of weight and the alcohol immersion of volume fraction 95% supersound process 20 to 60 minutes, after filtration, residue is with the distilled water immersion that newly boils and cool of 1-2 times of weight and be placed in refrigerator-15 DEG C to-25 DEG C of freezing 8-12 hour, taking-up is placed on 800 watts of processing 20-40 minute in microwave oven, stopped once every 5 minutes, 5-10 minute/time, then filter, residue is again with the distilled water immersion that newly boils and cool of 1-2 times of weight, repeated freezing and microwave processing process 2-5 time, merging filtrate is polysaccharide extraction liquid,(2) purifying;

    with pretreated 50 ×

    1.5 centimetres of glass columns of macroporous adsorbent resin filling, saturated with distilled water, aforesaid polysaccharide extraction liquid normal pressure is crossed after post, be evaporated to the 1/5-1/2 of original volume, the ethanol that is 95% by the volume fraction of 5-6 times of volume again precipitation, by throw out lyophilize, then use successively acetone, anhydrous diethyl ether and absolute ethanol washing, volatilize solvent and can obtain pure Herba Cistanches polysaccharide.

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