Nucleic acid screening method of staphylococcus aureus, salmonella, shigella and listeria monocytogenes

Nucleic acid screening method of staphylococcus aureus, salmonella, shigella and listeria monocytogenes

  • CN 101,880,711 B
  • Filed: 03/11/2010
  • Issued: 04/19/2017
  • Est. Priority Date: 03/11/2010
  • Status: Active Grant
First Claim
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1. a kind of nucleic acid detection method for staphylococcus aureuses, only relates to the examination of non-diagnostic purpose, is mediated using ringIsothermal duplication (LAMP) technology is carrying out, it is characterised in that by using specific primer, using LAMP technology platform target is expandedThe specific region of gene, under the auxiliary of positive and negative Quality Control and internal reference detection system, from molecular level to golden yellow PortugalGrape coccus carries out nucleic acid screening or detection;

  • Described staphylococcus aureuses clfA genes LAMP detects that specific primer is:

    The LAMP reaction primer sequence compositions of (GeneBank EF207779)

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