Semi-recombinant preparation of GLP-1 analogues

Semi-recombinant preparation of GLP-1 analogues

  • CN 101,910,193 A
  • Filed: 12/22/2008
  • Published: 12/08/2010
  • Est. Priority Date: 12/28/2007
  • Status: Active Application
First Claim
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1. one kind is used to prepare in the N end parts and comprises the GLP-1 analogue of one or more non-protein amino acids or the method for derivative, said method comprising the steps of:

  • I) host cell of the nucleotide sequence of the precursor molecule that comprises the described GLP-1 analogue of encoding is cultivated being used to express under the felicity condition of described precursor molecule,Ii) from liquid nutrient medium, isolate the precursor molecule of expression,Iii) will comprise the N terminal amino acid overhang of one or more non-protein amino acids and the precursor molecule coupling of expression,Iv) GLP-1 analogue or the derivative by suitable method resulting separation.

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