The method of efficient extraction of protein from cell

The method of efficient extraction of protein from cell

  • CN 101,910,400 B
  • Filed: 11/14/2008
  • Issued: 06/27/2017
  • Est. Priority Date: 11/14/2007
  • Status: Active Grant
First Claim
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1. the method for being used to produce protein extract from fixed or loose cell, methods described includes:

  • A) cell is made to be in contact with extracts reagent, so that produce with least intermediate composition of the pH of pH 10.0, andB) intermediate composition is in contact with neutralization reagent, so as to neutralize the pH of the intermediate composition, and produceThe protein extract,Wherein, one or both of the extracts reagent and the neutralization reagent contain polyoxyethylene alkyl ether.

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