Detection of methicillin-resistant staphylococcus aureus

Detection of methicillin-resistant staphylococcus aureus

  • CN 101,918,593 A
  • Filed: 12/19/2008
  • Published: 12/15/2010
  • Est. Priority Date: 12/21/2007
  • Status: Active Grant
First Claim
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1. the method for methicillin resistant Staphylococcus aureus (MRSA) in the test sample, described methicillin resistant Staphylococcus aureus has the insertion of SCCmec box in the streptococcus aureus chromosomal DNA, and described method comprises:

  • Utilize following material that sample is increased and detection reaction;

    (a) first primer, it can be hybridized at the terminal joining region of SCCmec box internal specific,(b) second primer, its can the terminal joining region of streptococcus aureus chromosomal DNA internal specific hybridization and(c) probe, its can and the zone that can hybridize with it of first primer and the hybridization of the SCCmec box regiospecificity between the joining region,Wherein each first primer and second primer are oriented to;

    make under amplification condition, described joining region is amplified, andIf wherein contain MRSA in the sample, then detect the hybridization of probe.

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