Porcine circovirus disease detection method based on ORF2 (Open Reading Frame 2) antigen domain

Porcine circovirus disease detection method based on ORF2 (Open Reading Frame 2) antigen domain

  • CN 101,936,993 A
  • Filed: 07/24/2010
  • Published: 01/05/2011
  • Est. Priority Date: 07/24/2010
  • Status: Active Application
First Claim
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1. the method based on the detection pig circular ring virus 2 in ORF2 antigen function district is characterized in that comprising the steps:

  • The design Auele Specific Primer, amplification obtains the genetic fragment of the amino acid section 113-147aa on the ORF2;

    Target gene fragment is cloned among the pGEX-4T-1 abduction delivering GST-ORF2-E fusion in e. coli bl21 (DE3) bacterial strain, and purifying;

    With purifying protein as antigen coated 96 hole elisa plates;

    Add serum to be checked;

    Add two of HRP mark and resist, be i.e. the anti-pig IgG of HRP mark;

    Adding hydrochloric acid o-phenylenediamine (OPD) quick colour-developing liquid develops the color;

    Measure the optical density (OD of 490nm 490) and sentence read result.

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