There are the polypeptide of lipase activity and the polynucleotide of this polypeptide of coding

There are the polypeptide of lipase activity and the polynucleotide of this polypeptide of coding

  • CN 101,960,008 B
  • Filed: 02/26/2009
  • Issued: 04/13/2016
  • Est. Priority Date: 02/29/2008
  • Status: Active Grant
First Claim
Patent Images

1. there is a polypeptide for lipase activity, described polypeptide be have following in the polypeptide of at least one:

  • A () is less than 500LU/A280 relative to the lipase activity (LU) of the absorbancy (A280) at 280nm place, wherein be defined as can at 30 DEG C for the LU (1LU) of a unit, pH7 per minute discharges the enzyme amount of the butyric acid of 1 μ

    m of ol, and the absorbancy of described polypeptide measures at 280nm place;

    B () risk performance smell (R) is lower than 0.5, wherein R is calculated as from the butyric acid amount of polypeptide washing sample release and the ratio from the butyric acid amount with reference to the release of polypeptide washing sample, and the first two value of calculating ratio all corrects relative to the butyric acid amount from the release of non-polypeptide washing sample;

    OrC () Benefit Risk factor (BR) is at least 1.8, wherein BR is defined as average scourability (RP avg) divided by risk performance smell (R), Described polypeptide be the combination comprising following mutational site on the aminoacid sequence basis such as shown in SEQIDNO;

    2;

    T231R+N233R+I255A+P256K and following at least one;

    (a) S58A+V60S+A150G+L227G;

    Or(b)E210V/G;

    Wherein said polypeptide also comprises the change that at least one is selected from lower group;

    E1N/*, D27N, N33Q, S83T, G91N, N94R, K98I, E99K, D102A, D111N, G163K, E210A, S216P or L259F of SEQIDNO;

    2.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×