Method for amplifying locus in bacterial cell

Method for amplifying locus in bacterial cell

  • CN 101,981,193 A
  • Filed: 03/27/2009
  • Published: 02/23/2011
  • Est. Priority Date: 03/28/2008
  • Status: Active Application
First Claim
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1. the method for amplification gene seat, it comprises:

  • (a) will comprise structure is A 1-P-M-A 2The colony of bacterial host cell of locus contact A wherein with the inhibitor of indispensable enzyme 1And A 2Be to repeat in the same way, P comprises the encoding sequence of target protein matter, and M comprises the encoding sequence of described indispensable enzyme;

    With (b) select cell that described inhibitor is had resistance, wherein the cell that described inhibitor is had a resistance has the described amplification unit of a plurality of copies.

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