The method of amplifying locus in bacterial cell

The method of amplifying locus in bacterial cell

  • CN 101,981,193 B
  • Filed: 03/27/2009
  • Issued: 05/11/2016
  • Est. Priority Date: 03/28/2008
  • Status: Active Grant
First Claim
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1. the method for amplifying locus, it comprises:

  • (a) will comprise that to contain structure be A1-P-M-A2Colony and the indispensable enzyme of bacterial host cell of locus of amplification unitInhibitor contact, wherein A1And A2To repeat in the same way, coded sequence that P comprises destination protein matter, M comprises described indispensable enzymeCoded sequence;

    With(b) select that described inhibitor is had to the cell of resistance, the cell that wherein described inhibitor is had to a resistance has multipleCopy described amplification unit.

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