Multiplex cell signalling assay

Multiplex cell signalling assay

  • CN 102,016,580 B
  • Filed: 01/15/2009
  • Issued: 10/13/2017
  • Est. Priority Date: 01/18/2008
  • Status: Active Grant
First Claim
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1. the method for measuring at least one intracellular events and cell analyte of interest in single living cell group, wherein instituteIt is the collaboration Biochemical processes run in the cell to state at least one intracellular events and the cell analyte of interestEach component, the described method comprises the following steps:

  • A) sample containing single living cell group is provided;

    B) at least one living cells allowed in the individual cells group causes at least one described cell to produce with causing or suspectingThe detection reagent contact of cell analyte of interest;

    C) at least one described cell physical properties change, be used as the measurement of at least one intracellular events;

    D) cell is washed to remove extracellular liquid;

    E) immunocytochemical determination interacted by protein-antibody or peptide-antibody measures the cell correlation analysisThe presence of thing or content;

    WithF) by the change of at least one intracellular events at least one described cell and the cell analyte of interestPresence or content be associated,Wherein intracellular events are ion concentration increase and/or gene expression increase;

    Wherein described cell analyte of interest refer to after cell is stimulated generally by the biochemical process or approach that cooperate with produce it is thinBorn of the same parents'"'"' component, this component can then be secreted by cell, but measure the presence of cell analyte of interest or itself and cell during contentPhysically it is connected;

    Wherein described cell analyte of interest is the cell analyte of interest combined with cell membrane;

    AndWherein described cell analyte of interest is growth factor, cell factor, chemotactic factor (CF) or integrin.

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