Amplification of nucleic acids using temperature zones

Amplification of nucleic acids using temperature zones

  • CN 102,076,869 A
  • Filed: 06/16/2009
  • Published: 05/25/2011
  • Est. Priority Date: 06/23/2008
  • Status: Active Application
First Claim
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1. be used for increasing and the amplification solution of detection reaction container in the method for target nucleic acid sequence, comprise the steps:

  • Reaction vessel is provided, and this reaction vessel comprises at least one hybridization zone, surface of immobilized capture probes from the teeth outwards, and the zone on wherein said capture probe and the described target nucleic acid sequence is complementary substantially;

    Add amplification solution to described reaction vessel;

    Generation has the temperature province distribution plan in the reaction vessel at least two kinds of hot decoupling zones, and wherein a kind of zone is identical with hybridization zone, surface or overlap at least, and wherein hybridization zone in surface has the generation temperature that allows capture probe to hybridize to the target nucleic acid sequence;

    In reaction vessel, carry out the amplification of target nucleic acid sequence;

    AndDuring increasing and/or afterwards detect nucleotide sequence through amplification termly or with the timed interval that limits, wherein the nucleotide sequence through amplification detects by the hybridization of capture probe in hybridization zone, described surface with described nucleotide sequence through increasing.

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