High throughput nucleic acid sequencing by expansion

High throughput nucleic acid sequencing by expansion

  • CN 102,083,998 A
  • Filed: 06/19/2008
  • Published: 06/01/2011
  • Est. Priority Date: 06/19/2007
  • Status: Active Application
First Claim
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1. be used for the method for target nucleic acid order-checking, comprise:

  • A) provide by the template guided synthetic subchain that is produced, described subchain comprises a plurality of subunits that are coupled in the sequence, described sequence is corresponding to the continuous nucleotide sequence of all or part of described target nucleic acid, wherein individual subunit comprises connecting arm, at least one probe or nuclear base residue, but and at least one selectivity shear key;

    B) but shear at least one selectivity shear key, produce the length Xpandomer longer than a plurality of subunits of subchain, described Xpandomer comprises described connecting arm and is used for resolving the report element of the genetic information of sequence, and described sequence is corresponding to the continuous nucleotide sequence of all or part of described target nucleic acid;

    AndC) the report element of the described Xpandomer of detection.

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