Method for electrochemically identifying target nucleotide sequences

Method for electrochemically identifying target nucleotide sequences

  • CN 102,112,630 A
  • Filed: 06/04/2009
  • Published: 06/29/2011
  • Est. Priority Date: 06/05/2008
  • Status: Active Application
First Claim
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1. be used for electrochemistry and identify the method for target nucleotide sequences, described method is according to following type:

  • -biological sample is provided, wherein can comprise predetermined target nucleotide sequences;

    But-provide the activated that comprises free nucleotide amplification device, with the formation of the target nucleotide sequences of duplicating and duplicating that causes described predetermined target nucleotide sequences;

    -provide can with the oxidable reductive compound of described Nucleotide reaction, and described oxidable reductive compound is contacted with described biological sample;

    The described amplification device that activates of-activation;

    -described sample is applied electric field activating described oxidable reductive compound, and measure the electric current of the electrochemical activity of the described oxidable reductive compound of representative, described electric current is through described sample;

    WithIf-current decay then determines to exist described predetermined target nucleotide sequences;

    Described method is characterised in that to be provided and can form the oxidable reductive compound that inserts between the Nucleotide of the described target sequence that duplicates between replicative phase, the described target sequence that duplicates causes the inhibition of electrochemical activity of the oxidable reductive compound of described insertion, current decay thus.

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