Cell storage method and cell transport method

Cell storage method and cell transport method

  • CN 102,203,241 B
  • Filed: 10/23/2009
  • Issued: 05/03/2017
  • Est. Priority Date: 10/24/2008
  • Status: Active Grant
First Claim
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1. cell preservation method, it is to preserve hepatocellular cell using the cell culture container with multiple micro- spaces to preserveMethod,Including,Above-mentioned micro- space is formed by micro- container,Four sides of above-mentioned micro- container are fully surrounded by side wall,Culture processes:

  • The surface that liver cell adheres to above-mentioned multiple micro- containers is cultivated, is formed in each above-mentioned micro- containerThree-dimensional structure,Preserve preparatory process:

    After above-mentioned culture, remove and inject culture medium to above-mentioned cell culture container after non-adherent cellAnd the preparation for being preserved, to cover above-mentioned multiple micro- containers,Preserve operation:

    By the sealing of above-mentioned cell culture container, in the state of above-mentioned three-dimensional structure is maintained, so that culture medium is notSpill, preserve above-mentioned liver cell,Above-mentioned cell culture container is maintained at 10 DEG C less than 20 DEG C of temperature preserving above-mentioned liver cell,In above-mentioned culture processes, the above-mentioned three-dimensional structure in each above-mentioned micro- container above-mentioned side wall and other above-mentioned three-dimensionalsStructure is isolated, so that above-mentioned three-dimensional structure does not connect with adjacent above-mentioned three-dimensional structure,Above-mentioned three-dimensional structure is the globuli cell group that above-mentioned liver cell gathers.

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