Method for determining nucleic acids by real-time polymerase chain reaction and a device for the implementation thereof

Method for determining nucleic acids by real-time polymerase chain reaction and a device for the implementation thereof

  • CN 102,203,272 A
  • Filed: 10/08/2009
  • Published: 09/28/2011
  • Est. Priority Date: 10/23/2008
  • Status: Active Application
First Claim
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1. identify the method for nucleic acid by real-time polymerase chain reaction for one kind, described method comprises:

  • the liquid sample that will contain nucleic acid is introduced in the conversion zone on the upper surface of thermal conductive substrate of microchip;

    With sample and the isolated from atmosphere of being introduced;

    Come at the outside surface by described microchip under the situation of heat extraction, the nucleic acid with described sample in the thermal cycling process of described sample contacts with the composition of described polymerase chain reaction;

    Fluoroscopic examination is carried out in variation to the amount of described polymerase chain reaction product in the thermal cycling process;

    The kinetics of the growth by fluorescent signal is identified original nucleic acid amount in the described sample, and wherein, described microchip and thermal conductivity are higher than 1W/cmK and thermal diffusivity is higher than 0.6cm 2The described thermal conductive substrate of/s is used together, the lip-deep described conversion zone of described microchip separates by layer of passivation material and the described thermal conductive substrate that is covalently attached to described thermally conductive material surface simultaneously, and the sample of described introducing is by being separated itself and atmosphere and isolated by the water-immiscible liquid layer, described liquid level remains on the upper surface of described thermal conductive substrate by outer surrounding baffle, and the ratio of total heat storage capacity and the thermal conductivity of described microchip substrate that has the described microchip of the sample introduced and described water-immiscible liquid layer is no more than 0.04s.

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