Method for transfecting pig T lymphocytes in vitro by applying nuclear transfection method

Method for transfecting pig T lymphocytes in vitro by applying nuclear transfection method

  • CN 102,206,678 A
  • Filed: 04/12/2011
  • Published: 10/05/2011
  • Est. Priority Date: 04/12/2011
  • Status: Active Application
First Claim
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1. use consideration convey and dye the outer lymphocytic method of transfection pig T of body of laws, comprise the steps:

  • that the nucleus transfection liquid of using electroporation technology and cell-specific will contain in the mRNA importing pig T lymphocyte of expression of exogenous gene carrier or foreign gene, the cell after the transfection is through cultivating, sub-elect the pig T lymphocyte of expression alien gene.

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