Nitrile hydratase variant

Nitrile hydratase variant

  • CN 102,216,455 B
  • Filed: 11/12/2009
  • Issued: 05/04/2016
  • Est. Priority Date: 11/14/2008
  • Status: Active Grant
First Claim
Patent Images

1. a nitrile hydratase variant, is characterized in that, comprises at least 1 amino acid and getsOn behalf of other amino acid whose replacements, described replacement is by 3 following 1 above amino acidReplace and improve 2 of nitrile hydratase above character,Described character is initial velocity of reaction and heat endurance,Described nitrile hydratase variant contains the sequence number of ordered list:

  • 1 alpha subunit representingSequence number with sequence table;

    the 2 β

    subunits that represent, and following (I), (II) or(III) mutable site shown in is by other 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factors;

    (I) the 92nd of alpha subunit the,(II) the 92nd of alpha subunit the, and the 226th of β

    subunit,(III) the 92nd of alpha subunit the, and the 4th and the 206th of β

    subunit,Mutable site shown in described (I) is during by other 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factors,Be substituted by Glu by the 92nd of alpha subunit, and, following (a), (b) comprisedOr the 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor of the mutable site (c) is other amino acid whose replacements;

    (a) the 36th, 148 and 204 of alpha subunit, and β

    subunit the 41st,51 and 108,(b) the 36th of alpha subunit the, and the 33rd of β

    subunit,(c) the 40th of β

    subunit the and 61,When the 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor of the mutable site shown in described (a) is other amino acid,Be substituted by Met by the 36th of alpha subunit;

    Be substituted by Asp by the 148th of alpha subunit;

    Be substituted by Arg by the 204th of alpha subunit;

    Be substituted by Ile by the 41st of β

    subunit;

    Be substituted by Val by the 51st of β

    subunit;

    AndBe substituted by Asp by the 108th of β

    subunit,When the 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor of the mutable site shown in described (b) is other amino acid,Be substituted by Ser by the 36th of alpha subunit;

    AndBe substituted by Val by the 33rd of β

    subunit,When the 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor of the mutable site shown in described (c) is other amino acid,Be substituted by Ile by the 40th of β

    subunit;

    AndBe substituted by Val by the 61st of β

    subunit,Mutable site shown in described (II) is during by other 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factors,Be substituted by Glu by the 92nd of alpha subunit;

    AndBe substituted by Ile by the 226th of β

    subunit,And, comprise following (d), (e), (f) or (g) shown in mutable site49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor is other amino acid whose replacements;

    (d) the 41st, 51 and 108 of β

    subunit,(e) the 148th of alpha subunit the and 204, and β

    subunit the 108th and 200Position,(f) the 61st of β

    subunit the and 112,(g) the 24th, 41,51 and 108 of β

    subunit,When the 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor of the mutable site shown in described (d) is other amino acid,Be substituted by Ile by the 41st of β

    subunit;

    Be substituted by Val by the 51st of β

    subunit;

    AndBe substituted by Asp by the 108th of β

    subunit,When the 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor of the mutable site shown in described (e) is other amino acid,Be substituted by Asp by the 148th of alpha subunit;

    Be substituted by Arg by the 204th of alpha subunit;

    Be substituted by Asp by the 108th of β

    subunit;

    AndBe substituted by Glu by the 200th of β

    subunit,When the 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor of the mutable site shown in described (f) is other amino acid,Be substituted by Leu by the 61st of β

    subunit;

    AndBe substituted by Ile by the 112nd of β

    subunit,When the 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor of the mutable site shown in described (g) is other amino acid,Be substituted by Ile by the 24th of β

    subunit;

    Be substituted by Ile by the 41st of β

    subunit;

    Be substituted by Val by the 51st of β

    subunit;

    AndBe substituted by Asp by the 108th of β

    subunit,Mutable site shown in described (III) is during by other 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factors,Be substituted by Glu by the 92nd of alpha subunit;

    Be substituted by Met by the 4th of β

    subunit;

    AndBe substituted by Leu by the 206th of β

    subunit,And, comprise following (h), (i) or (j) shown in the amino acid of mutable siteBe substituted by other amino acid whose replacements;

    (h) the 148th of alpha subunit the and 204,(i) the 36th, 148 and 204 of alpha subunit,(j) the 51st of β

    subunit the and 108,When the 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor of the mutable site shown in described (h) is other amino acid,Be substituted by Asp by the 148th of alpha subunit;

    AndBe substituted by Arg by the 204th of alpha subunit,When the 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor of the mutable site shown in described (i) is other amino acid,Be substituted by Met by the 36th of alpha subunit;

    Be substituted by Asp by the 148th of alpha subunit;

    AndBe substituted by Arg by the 204th of alpha subunit,When the 49-Phe ,82-Ser,115-Arg,144-Met,145-Asn ,161-Arg,169-Met Human Connective tissue growth factor of the mutable site shown in described (j) is other amino acid,Be substituted by Val by the 51st of β

    subunit;

    AndBe substituted by Asp by the 108th of β

    subunit.

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