Method of producing D-serine

Method of producing D-serine

  • CN 102,226,207 A
  • Filed: 10/07/2005
  • Published: 10/26/2011
  • Est. Priority Date: 10/13/2004
  • Status: Active Application
First Claim
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1. the preparation method of a D-Serine, described preparation method is in the presence of having by the active microorganism of the synthetic D-Serine of glycine and formaldehyde or its handled thing, make glycine and formaldehyde reaction synthesize the D-Serine, described handled thing is selected from described microorganism is carried out physical disturbance, ultrasonication, freeze to melt processing, drying treatment, pressurization or reduced pressure treatment, osmotic pressure is handled, autodigestion, tensio-active agent is handled, enzyme is handled and the material that obtains, and by resulting fixed compound and the described microbial immobilization thing that has by the part of the active enzyme of the synthetic D-Serine of glycine and formaldehyde that contain of above-mentioned processingWherein, it is 1.5 moles below the % that the method that adopts in following (1)~

  • (4) more than one is suppressed at secondary L-Serine of giving birth in the reaction solution of described reaction with respect to the D-Serine,(1) the active microorganism that has by glycine and the synthetic D-Serine of formaldehyde is carried out organic solvent processing and/or methods of heating treatment;

    (2), when a) carrying out organic solvent processing and/or heat treated, be controlled at below the 2M by the method for described (1) with the concentration of formaldehyde in the reaction solution;

    At b) when not carrying out organic solvent processing and/or heat treated, be controlled at the following method of the above 2M of 150mM;

    (3) use to contain and have by the microorganism of the active enzyme of glycine and the synthetic D-Serine of formaldehyde and L-Serine synthase gene disappearance method as catalyzer;

    (4) interpolation contains the method for microorganism with the active enzyme of L-serine deaminase in reaction solution.

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