Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum

Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum

  • CN 102,227,502 A
  • Filed: 09/30/2009
  • Published: 10/26/2011
  • Est. Priority Date: 09/30/2008
  • Status: Active Application
First Claim
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1. method that produces polypeptide comprises:

  • (a) cultivate the mutant of parent'"'"'s empiecement sickle spore (Fusarium venenatum) bacterial strain at the substratum that is used for producing described polypeptide, one or more (several) gene;

    pyrG that wherein said mutants which had comprises the polynucleotide of coding said polypeptide and is selected from down group, amyA and alpA, wherein said one or more (several) gene is modified to make described mutants which had compare defectiveness in the generation that is selected from one or more (several) enzyme of organizing down respectively with parent'"'"'s empiecement sickle spore bacterial strain of cultivating under the same conditions;

    orotidine-5 '"'"'-single phosphate decarboxylase, α

    -Dian Fenmei and Sumizyme MP;

    With(b) reclaim described polypeptide from substratum.

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