Methods for identifying immunobinders of cell-surface antigens

Methods for identifying immunobinders of cell-surface antigens

  • CN 102,239,181 A
  • Filed: 02/22/2010
  • Published: 11/09/2011
  • Est. Priority Date: 02/24/2009
  • Status: Active Application
First Claim
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1. be used to identify the method for the immunoconjugator of specificity binding purposes cell-surface antigens, it comprises:

  • (a) but a plurality of cells that may be operably coupled to the expression immunoconjugator of first sorting indicia are provided;

    (b) but a plurality of cells that may be operably coupled to the antigen expressed of second sorting indicia are provided, wherein purpose antigen is illustrated in the surface of the cell of antigen expressed;

    (c) with the cell of antigen expressed and the cells contacting of expressing immunoconjugator;

    With(d) use cell sorter from the cell of the one or more energy of the cellular segregation of a plurality of expression immunoconjugators specificity in conjunction with the expression immunoconjugator of expressing antigenic cell, but the existence of wherein said first and second sorting indicia in the individual cells mixture indicating the cell of expressing immunoconjugator and combining with the cell of antigen expressed, thereby identified the antigenic immunoconjugator of binding purposes.

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