Method for preparing CysC-paired monoclonal antibody

Method for preparing CysC-paired monoclonal antibody

  • CN 102,321,176 A
  • Filed: 08/31/2011
  • Published: 01/18/2012
  • Est. Priority Date: 08/31/2011
  • Status: Active Application
First Claim
Patent Images

1. one kind prepares bladder chalone C pairing monoclonal antibody method, it is characterized in that may further comprise the steps:

  • After can producing the mouse boosting cell and myeloma cell'"'"'s fusion of bladder chalone C antibody;

    Screening has specificity bonded hybridoma with bladder chalone C, and the cell of monoclonal antibody is produced in preparation, accomplishes cytogamy the 1st time;

    Be further purified out monoclonal antibody, select 1 monoclonal antibody mark horseradish peroxidase;

    Implementing the 2nd mouse boosting cell and myeloma cell then merges;

    It is antigen coated on enzyme plate, to accomplish bladder chalone C;

    The Hybridoma Cell Culture supernatant that the 2nd cytogamy obtained is added in the enzyme plate hole, and establishes the blank hole, and the monoclonal antibody with above-mentioned horseradish peroxidase-labeled is added in the enzyme plate hole subsequently;

    37 ℃

    of incubations 30 minutes;

    Every hole adds substrate develops the color, and after the sulfuric acid termination reaction, measures the light absorption value under the 450nm;

    The hole of choosing unrestraint is as the pairing monoclonal antibody.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×