Vaccine taking HBeAg egg white and/or HBeAg epitope polypeptide as immunogen

Vaccine taking HBeAg egg white and/or HBeAg epitope polypeptide as immunogen

  • CN 102,327,611 B
  • Filed: 09/14/2011
  • Issued: 04/26/2017
  • Est. Priority Date: 09/14/2011
  • Status: Active Grant
First Claim
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1. Application of the 1.HBeAg antigen epitope polypeptides as immunogen in vaccine is prepared, it is characterised in that the vaccine is by makingMake for immunogenic HBeAg antigen epitope polypeptides mix with adjuvant;

  • Described HBeAg antigen epitope polypeptides are SEQ ID NO;

    2~

    SEQ ID NO;

    One or more shown in 11;

    Described adjuvant is aluminium adjuvant, nano-class aluminum adjuvant, PLA-PEG copolymer, poly lactic-co-glycolic acid, keyholeLimpet hemocyanin, shitosan or oil phase;

    Described adjuvant is aluminium adjuvant, and preparation is the AL (OH) by 0.1~

    10 times of HBeAg antigen epitope polypeptides and its quality3,Mix in the phosphate buffer of pH6.8, add AL (OH)3The Mannitol that 0.1~

    2 times of quality, sucrose, albumin, hydrolysisGelatin, Lactose or Sorbitol are mixed;

    Described adjuvant is PLA-PEG copolymer, and preparation is by double emulsion systems by HBeAg antigen epitope polypeptidesPLA-PEG copolymer is adsorbed in, the Nano microsphere that particle diameter is 0.5~

    10 μ

    m, polylactic acid-polyglycol copolymerization is formedThing drug loading is more than 80% more than 15%, envelop rate;

    Described adjuvant is nano-class aluminum adjuvant, and preparation is that HBeAg antigen epitope polypeptides are adsorbed in particle diameter is receiving for 50~

    100nmRice Al (OH)3In the middle of colloidal sol, HBeAg antigen epitope polypeptides:

    Nanometer Al (OH)3Mass ratio=10~

    20 μ

    the g of colloidal sol:

    1mg, nanometerAl(OH)3The adsorption rate of colloidal sol is more than 10 μ

    g/1mg;

    Described adjuvant is oil phase, and preparation is that HBeAg antigen epitope polypeptides are scattered in oil phase, then make particle diameter for 10~

    The water-in-oil type nano-particle of 30nm, oil phase are 1 with the volume ratio of water phase;

    3~

    5;

    Oil phase is Span-20;

    poloxamer188;

    Soybean oil=80;

    180;

    100 mass ratio mixing, water are mutually distilled water;

    The quality of HBeAg antigen epitope polypeptides and oil phaseThan for 1;

    200~

    300;

    Described adjuvant is poly lactic-co-glycolic acid, and preparation is that HBeAg antigen epitope polypeptides is common with poly lactic-co-glycolic acidPolymers is according to 1;

    20~

    50 mass ratio stirring and emulsifying in organic solvent, forms water-in-oil-in water emulsion, then under room temperatureOrganic solvent is removed, the microsphere that the particle diameter obtained by lyophilization is 100~

    400nm is washed with water after washing, envelop rate is more than40.0%;

    Described adjuvant is shitosan, and preparation is that HBeAg antigen epitope polypeptides are adsorbed in chitosan particle, and forming particle diameter isThe nano-particle of 100~

    200nm, HBeAg antigen epitope polypeptides:

    The mass ratio 1 of shitosan;

    3~

    5;

    Described adjuvant is keyhole limpet hemocyanin, and preparation is that HBeAg antigen epitope polypeptides are coupled to keyhole limpet blood indigo plantAlbumen, HBeAg antigen epitope polypeptides:

    The mass ratio 1 of keyhole limpet hemocyanin;

    3000~

    2;

    30.

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