The preparation method of a kind of Myricitroside and pharmaceutical composition thereof

The preparation method of a kind of Myricitroside and pharmaceutical composition thereof

  • CN 102,329,355 B
  • Filed: 07/05/2011
  • Issued: 09/02/2015
  • Est. Priority Date: 07/05/2011
  • Status: Active Grant
First Claim
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1. a preparation method for Myricitroside, is characterized in that, comprises the steps:

  • that a. extracts crude extract;

    with Cortex Myricae Rubrae 500g for raw material, 95% ethanol is as solvent, flood under room temperature and raw material is extracted, merging filtrate, concentrating under reduced pressure dry desolventizing obtains crude extract dry powder 48g;

    B. separation, purifying;

    crude extract high-speed countercurrent chromatography is separated, purifying, obtains Myricitroside monomer;

    The solvent systems of described high-speed countercurrent chromatography is petroleum ether-ethyl acetate-methanol-water, and ratio is 3;

    4;

    4;

    3, and concrete steps are;

    1. by crude extract 500mg with dissolve with methanol, and fixed molten 0.1g/ml, homogeneous clear state, by the solvent systems petroleum ether-ethyl acetate-methanol-water configured in proportion, two phase solvent system, above as stationary phase, make moving phase mutually down;

    2. pumped under Peak Flow Rate by upper phase solvent and be full of centrifuge tube cylinder, then lower phase solvent being pumped into centrifuge tube cylinder with the flow velocity of 5ml/min, open detection device is also by selected rotating speed 850 rpms simultaneously, starts main frame;

    3. flow out until moving phase from tubing string outlet and injected by sample introduction circle by sample solution after baseline stability, the effluent in tubing string exit detects through UV-detector and by chromatographic working station and registering instrument record, collects component.

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