Same sequence primer transpositional nucleic acid amplification technology

Same sequence primer transpositional nucleic acid amplification technology

  • CN 102,352,350 A
  • Filed: 09/30/2011
  • Published: 02/15/2012
  • Est. Priority Date: 09/30/2011
  • Status: Active Application
First Claim
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1. one kind with preface primer metathetical nucleic acid amplification technologies, it is characterized in that its step comprises:

  • (1) connects artificial sequence at the target special primer 5 '"'"' upper reaches, form chimeric primers;

    (2) produce the secondary template of two ends band artificial sequence with said chimeric primers amplified target template;

    (3) with 65%-75% with the artificial sequence of preface as displacement primer amplification secondary template.

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