Methods for the detection and identification of extended spectrum beta lactamases

Methods for the detection and identification of extended spectrum beta lactamases

  • CN 102,388,150 A
  • Filed: 02/19/2010
  • Published: 03/21/2012
  • Est. Priority Date: 02/19/2009
  • Status: Active Application
First Claim
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1. be used for detecting with or identify that sample has the method that the mikrobe of CTX-M extended spectrum exists, it comprises:

  • From the sample of the existence of CTX-M gene to be analyzed, obtain sample;

    Under Standard PC R condition, said sample is contacted with the amplimer collection, wherein;

    It is right that said amplimer collection comprises at least one pair of primer, and wherein, said primer collection comprises more than one primer with universal base;

    Wherein, Said primer pair and the nucleic acid hybridization that is positioned at the intragenic target sequence flank of CTX-M extended spectrum, and wherein, said primer is to producing the target amplified production;

    Be provided for the reagent and the condition of primer extension, to produce the target amplified production;

    AndConfirm the existence and/or the amount of target amplified production, wherein, the existence of said CTX-M gene is explained in the existence of said target amplified production.

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