Multi-mode molecular imaging monitoring method of ischemia model

Multi-mode molecular imaging monitoring method of ischemia model

  • CN 102,389,297 A
  • Filed: 09/02/2011
  • Published: 03/28/2012
  • Est. Priority Date: 09/02/2011
  • Status: Active Application
First Claim
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1. the multi-modal molecular imaging monitoring method of ischemia model, it is characterized in that:

  • described monitoring step is;

    (1) stem cell of stably express luciferase and green fluorescent protein separates and cultivation;

    (2) make up the ischemic animal model;

    (3) transplant the ischemic injuries zone that the stem cell of cultivating in the step (1) arrives the constructed animal model of step (2);

    (4) be carried out to picture at the 1st day, the 7th day, the 14th day, the 21st day, the 28th day, the 35th day, the 42nd day with the bioluminescence computed tomography (SPECT) system, observation time point is till can'"'"'t see luminous stem cell;

    Thereby can locate stem cell petty action object internal memory three-dimensional space position and the survival of spike stem cell, through the stem cell quantitative approach observation stem cell survival quantity of different time points in vivo;

    (5) carry out the antibody staining of anti-green fluorescent protein through the stem cell of stably express green fluorescent protein;

    Put to death animal model and get muscular tissue making frozen section, do the existence checking of stem cell in ischemic tissue with fluorescence microscope in the zone of injection stem cell;

    (6) formed images with the blood capillary network of micro-CT imaging system to the animal ischemia model at the 1st day, the 7th day, the 14th day, the 21st day, the 28th day, the 35th day, the 42nd day, whether observation blood capillary network density variation has taken place;

    (7) verify the imaging results of micro-CT by the intervention group of the different time points after toy ischemia model and the stem cell transplantation and the blood vessel casting of matched group and the electron-microscope scanning result of blood vessel casting to blood capillary network variable density.

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