Improved culture method for preparing tissue cell with chromosome

Improved culture method for preparing tissue cell with chromosome

  • CN 102,443,563 A
  • Filed: 10/10/2010
  • Published: 05/09/2012
  • Est. Priority Date: 10/10/2010
  • Status: Active Application
First Claim
Patent Images

1. improvement Chromosome Preparation histocyte cultural method that is used for medical field;

  • Its principal character is design organization mill and anti-pollution culturing bottle, is used to improve classical Chromosome Preparation histocyte cultural method, after tissue block to be checked is put into tissue grinder;

    Only operated for 10~

    30 seconds;

    The cell that just can reach tissue culture disperses requirement, and promptly available conventional cell culture fluid suspension cell moves in the anti-contamination cell culturing bottle;

    Tighten bottle cap;

    Make conventional cell cultures Chromosome Preparation, therefore simple need shred nearly 2 hours operations such as tissue block, collagenase digesting, repeated washing, centrifugation and reagent preparation repeatedly in the classical way, and eliminated the pair cell physics that produces by classical way and the influence factors such as damage, experimental pollution and infection of chemistry;

    Anti-pollution culturing bottle can effectively prevent the bacterial contamination of cell cultivation process, can guarantee the CO that it is required again 2Content and humidity.

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