Multiple-biological material and preparation method thereof

Multiple-biological material and preparation method thereof

  • CN 102,458,495 B
  • Filed: 06/04/2010
  • Issued: 06/22/2016
  • Est. Priority Date: 06/04/2009
  • Status: Active Grant
First Claim
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1. a biomaterial, it has multidimensional structure and the MSC tissue comprising differentiation and demineralised bone matrix, wherein said demineralised bone matrix is dispersed in the MSC tissue of described differentiation, wherein said MSC is the stem cell of adipose tissue-derived, and the MSC tissue of wherein said differentiation is made up of chondroblast and/or osteoblast, described biomaterial comprises middle connective tissue, and there is the ability of angiogenesis in vivo, and wherein said biomaterial is obtained by the method comprised the following steps:

  • (i) hatches MSC in osteoblasts cultivation base and/or Subchondral drilling culture medium, then (ii) add in described osteoblasts cultivation base and/or Subchondral drilling culture medium demineralised bone matrix and maintain hatch and break up other a period of time。

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