Method and culture solution for amplification and culture of in-vitro serum-free adult stem cell

Method and culture solution for amplification and culture of in-vitro serum-free adult stem cell

  • CN 102,533,641 A
  • Filed: 02/20/2012
  • Published: 07/04/2012
  • Est. Priority Date: 02/20/2012
  • Status: Active Application
First Claim
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1. the method for an external serum-free adult stem cell amplification culture is characterized in that, this method comprises the following step:

  • Step 1;

    tissue is provided;

    Step 2;

    , and unhydrolysed tissue is separated with the human adult stem cell with centrifugation with the tissue of ferment hydrolysis step 1 gained;

    Step 3;

    the human adult stem cell of step 2 gained is carried out first culture in containing in the serum-free stem cell nutrient solution of the bulk-growth factor;

    Step 4;

    take out the type of the pasting cell in the first culture liquid in the step 3, carry out succeeding transfer culture, to accomplish human adult stem cell amplification culture in the serum-free stem cell nutrient solution that contains from the bulk-growth factor.

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