Fluorescence quantitative PCR detection kit and detection method for prawn infectious myonecrosis viruses

Fluorescence quantitative PCR detection kit and detection method for prawn infectious myonecrosis viruses

  • CN 102,559,484 A
  • Filed: 01/31/2012
  • Published: 07/11/2012
  • Est. Priority Date: 01/31/2012
  • Status: Active Grant
First Claim
Patent Images

1. prawn infectivity muscle necrosis virus fluorescent quantificationally PCR detecting kit comprises box body (1), is provided with pore support (5) in the box body (1), it is characterized in that being placed with on the pore support (5) following pipe fitting:

  • 1), quantitative fluorescent PCR reaction solution pipe (6), in adorn the mixed solution of 2 * quantitative fluorescent PCR buffer, the positive anti-primer of prawn infectivity muscle necrosis virus and fluorescently-labeled TaqMan probe;

    Forward primer sequence;

    5 '"'"'-GATGG CAGCA CGTCA AAATG-3 '"'"';

    Reverse primer sequence;

    5 '"'"'-CAGCT GTTCC TGGGA CTTCC T-3 '"'"';

    The TaqMan probe sequence;

    5 '"'"'-CTATT TCCTC CACTA ATTCA GAGC-3 '"'"', 5 '"'"' end of probe sequence is used the FAM mark, and 3 '"'"' end is used the TAMRA mark;

    2), de-ionized water pipe (3), interior dress aseptic deionized water;

    3), negative control pipe (4), interior dress SPF (no-special pathogen) prawn nucleic acid;

    4), standard QC (2), respectively in dress infectivity muscle necrosis virus 10 7, 10 6, 10 5, 10 4, 10 3, 10 2, 10,1 copy cDNA positive colonies.

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