A kind of deaf sick tumor susceptibility gene SLC26A4 IVS7-2A > G fluorescence detection reagent kit and application thereof

A kind of deaf sick tumor susceptibility gene SLC26A4 IVS7-2A > G fluorescence detection reagent kit and application thereof

  • CN 102,559,910 B
  • Filed: 02/14/2012
  • Issued: 02/24/2016
  • Est. Priority Date: 02/14/2012
  • Status: Active Grant
First Claim
Patent Images

1. a deaf sick tumor susceptibility gene SLC26A4IVS7-2A >

  • G fluorescence detection reagent kit, is characterized in that comprising the primer of archaeal dna polymerase and buffered soln, amplification IVS7-2A >

    G and Amelogenin locus and the genotype standard substance of gene mutation site IVS7-2A >

    G and Amelogenin locus and interior mark;

    the primer that described mutantional hotspot IVS7-2A >

    G detects is SEQIDNO.01 and SEQIDNO.02.2;

    SEQNO.01:

    5’

    -CCAGCATTGTAATTTTTTTCCAGG-3’



    SEQNO.02.2:

    5’

    -GTTTTTAACATCT TTGTTTTATTTAG-3’



    Wherein LNA nucleosides represents with boldface type;

    The described primer increased for IVS7-2A >

    G and Amelogenin, has 5 '"'"' of primer end to carry out fluorochrome label in often pair of primer.

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