Human blood coagulation factor VII I and the dry heat treatment stabilizer of vWF complex or human blood coagulation factor VII I preparation

Human blood coagulation factor VII I and the dry heat treatment stabilizer of vWF complex or human blood coagulation factor VII I preparation

  • CN 102,580,062 B
  • Filed: 03/09/2012
  • Issued: 03/08/2017
  • Est. Priority Date: 03/09/2012
  • Status: Active Grant
First Claim
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1. a kind of human blood coagulation factor VII I and the preparation of vWF complex or human blood coagulation factor VII I, are prepared by following methods:

  • 1)

    Cryoprecipitate is with 4 times of volume water dissolutioies, and adds anticoagulant sodium heparin, after room temperature extracts 30 minutes, with appropriate 3%-6% hydrogenOxidation aluminium glue is adsorbed, and is centrifuged after absorption, and supernatant crosses chromatographic column resin anion (R.A.) DEAE650 absorption, washs foreign proteinAfterwards, with 0.25M sodium chloride eluting, gained eluent carries out desalination and concentration by ultrafiltration, obtains human blood coagulation factor VII I and vWF and is combinedThing or human blood coagulation factor VII I preparation concentrated solution semi-finished product;

    2)

    Described concentrated solution half will be added to become without albuminous stabilizerIn product, after degerming, subpackage, lyophilizing, it is finished product through 100 DEG C of 30 minutes xeothermic inactivation of virus;

    Wherein said stabilizer is selected from concentrationOne of histidine for 1% or 2% or arginine that its salt, concentration are 1% or its salt.

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