Neutrophil gelatinase-associated lipocalin detection kit

Neutrophil gelatinase-associated lipocalin detection kit

  • CN 102,590,524 B
  • Filed: 12/30/2011
  • Issued: 01/06/2016
  • Est. Priority Date: 12/30/2011
  • Status: Active Grant
First Claim
Patent Images

1. a NGAL detection kit, comprises:

  • Reagent R1, reagent R2 and working calibration product;

    Wherein reagent R1 is NGAL and anti-human NGAL antibody response damping fluid;

    Reagent R2 is the Nano microsphere solution being combined with anti-human NGAL antibody;

    Working calibration product are made up of damping fluid, stabilizing agent, antiseptic and NGAL normative reference product,Comprise the Nano microsphere being combined with anti-human NGAL antibody of size two kinds of particle diameters in wherein said reagent R2, Large stone Nano microsphere is 220nm, and small particle diameter Nano microsphere is 74nm;

    Wherein said NGAL detection kit is selected from as follows;

    Kit 1;

    Reagent R1 consists of;

    pH7.3,

    Glycocoll 50mM,NaCl0.15M,

    NaN 30.1%



    EDTA10mM,

    BSA0.1%



    Rabbit igg 1%;

    R2 consists of;

    pH7.0,

    HEPES20mM,

    NaN 30.1%



    Small grain size latex content is;

    2%,Large grain size latex content is;

    0.5%,Working calibration product are;

    Buffer matrix, it is by HEPES50mM, NaCl0.15M, NaN 30.1%, EDTA10mM, BSA0.5%, sucrose 5%, all the other are deionized water composition, are dissolved in the NGAL normative reference product of the 5000ng/ml concentration of described matrix;

    Kit 2;

    Reagent R1 consists of;

    pH7.4,

    Glycocoll 50mM,NaCl0.25M,

    NaN 30.1%



    EDTA10mM,

    BSA0.1%



    R2 consists of;

    pH7.0HEPES20mM,

    NaN 30.1%



    Small grain size latex content is;

    2%,Large grain size latex content is;

    0.5%,Working calibration product are;

    Buffer matrix, it is by HEPES50mM, NaCl0.15M, NaN 30.1%, EDTA10mM, BSA0.5%, polysorbas20 0.1%, all the other are deionized water composition, are dissolved in the NGAL normative reference product of the 5000ng/ml concentration of described matrix;

    Kit 3;

    Reagent R1 consists of;

    pH7.4,

    Glycocoll 50mM,NaCl0.15M,

    NaN 30.1%



    EDTA10mM,

    BSA0.1%



    Mouse IgG1%,R2 consists of;

    pH7.0HEPES20mM,

    NaN 30.1%



    Small grain size latex content is;

    2%,Large grain size latex content is;

    0.5%,Working calibration product are;

    Buffer matrix, it is by HEPES50mM, NaCl0.15M, NaN 30.1%, EDTA10mM, BSA0.5%, sucrose 5%, all the other are deionized water composition, are dissolved in the NGAL normative reference product of the 5000ng/ml concentration of described matrix.

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