Method for purifying active GLA-domain coagulation proteins

Method for purifying active GLA-domain coagulation proteins

  • CN 102,666,857 A
  • Filed: 07/30/2010
  • Published: 09/12/2012
  • Est. Priority Date: 07/31/2009
  • Status: Active Application
First Claim
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1. the method for purifying GLA domain protein, it comprises the following steps:

  • The sample that a) will contain one or more GLA structural domain solidifying egg whites and possibly contain the biology non-activity molecule of GLA domain protein contacts with affine upholder;

    Fixed the aptamer that specificity combines the GLA structural domain solidifying egg white of at least a BA on the said affine upholder;

    Thereby between (i) said aptamer and (ii) said GLA structural domain solidifying egg white, form mixtureB) mixture that from step a), forms discharge GLA structural domain solidifying egg white andC) reclaim the GLA structural domain solidifying egg white of said BA with the form of purifying.

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