A kind of method extracting residual micronucleus acid from HE staining section

A kind of method extracting residual micronucleus acid from HE staining section

  • CN 102,680,295 B
  • Filed: 05/21/2012
  • Issued: 01/20/2016
  • Est. Priority Date: 05/21/2012
  • Status: Active Grant
First Claim
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1. one kind is extracted the method for residual micronucleus acid from HE staining section, it is characterized in that:

  • first make decolorant with low-concentration hcl ethanol and HE staining section is decoloured, then with oxalic acid aqueous solution bleaching, then enrich target cells, finally nucleic acid extraction is carried out to the target cell of enrichment;

    Concrete steps comprise;

    (1) HE staining section is immersed dimethylbenzene 30min and more than, gently slip out cover glass and take out, continuing to soak HE staining section 8 ~ 12h, more change to new dimethylbenzene, fully dispel neutral gum, obtain cell sheet;

    More change to new dimethylbenzene, soak 15 ~ 20min;

    (2) take out cell sheet soaked in absolute ethyl alcohol 10 ~ 20min, fully dispel dimethylbenzene;

    More change to new absolute ethyl alcohol, soak 15min, then carry out 95% alcohol immersion 10min successively;

    80% alcohol immersion 5min;

    70% alcohol immersion 2min;

    Wash 2min from the beginning;

    (3) with 1% hydrochloride alcohol haematoxylin decolouring more than cell sheet 30min;

    (4) 2% oxalic acid aqueous solutions soak cell sheet 3 ~ 10min;

    Tap water is slightly washed, distillation washing 30min, the target cell on enrichment of cell sheet, and forwards to immediately in centrifuge tube;

    (5) target cell nucleic acid is extracted;

    In operation overall process, forbid that cell sheet is in atmosphere exposed;

    Described HE staining section is;

    cut into slices by normal pathological process from surgery Pathology paraffin-embedded tissue, puncturing tissue, Gastrointestinal Endoscopes biopsy and cell smear, dye the HE staining section obtained.

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