Method for separating Morchella strains

Method for separating Morchella strains

  • CN 102,687,639 B
  • Filed: 12/19/2011
  • Issued: 05/13/2015
  • Est. Priority Date: 12/19/2011
  • Status: Active Grant
First Claim
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1. one kind is separated the method for hickory chick bacterial classification, it is characterized in that:

  • comprise the following steps;

    after the even wiping of hickory chick 75% ethanol for disinfection that adhesive tape is closed, above flame, fruit body head is cut away from stem top with the cutter burnt, the fresh stem cross section of exposing, briskly on flame to cross under 2-4, with cutter fresh meat bacteria organization of girdling stem send on antibiotic plating medium inside fresh stem, cultivate at the temperature of 20-30 DEG C, after sprouting mycelia, transfer in time;

    Morchella esculenta (L.) Pers sporophore wants base portion stem complete before cutting, and with transparent adhesive tape, whole for hickory chick winding is sealed, centre does not interspace;

    Described antibiotic plating medium is;

    potato 400g, peat composed of rotten mosses 100g, glucose 20g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, yeast extract 50g, agar 20g, water 1000mL, streptomycin 30U/mL, penicillin 30U/mL, pH 5-7.

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