Production method for an inactivated vaccine for type-C Clostridial enteritis

Production method for an inactivated vaccine for type-C Clostridial enteritis

  • CN 102,688,484 A
  • Filed: 06/13/2012
  • Published: 09/26/2012
  • Est. Priority Date: 06/13/2012
  • Status: Active Application
First Claim
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1. the production method of a chicken necrotizing enterocolitis (C type) inactivated vaccine;

  • It is characterized in that being inoculated in pancreas casein peptone and yeast extract powder by the volume ratio 1%~

    2% of culture medium total amount C type bacillus perfringens CVCC60102 strain seed liquor is in the liquid synthetic medium of main raw material(s);

    37 ℃

    of anaerobism cultivate/or leave standstill and cultivated 18 hours;

    Cultivate and finish after the centrifugal 30min of 3000r/min removes thalline, the millipore ultrafiltration and concentration system with the 8Ku molecular cut off carries out ultrafiltration and concentration respectively;

    After the formalin deactivation detoxification through 0.6% fully, the aluminium hydroxide gel that adds final concentration 20% is mixed with vaccine.

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