Preparation method for necrotic enteritis (Type A) inactivated vaccine

Preparation method for necrotic enteritis (Type A) inactivated vaccine

  • CN 102,698,259 B
  • Filed: 06/12/2012
  • Issued: 02/08/2017
  • Est. Priority Date: 06/12/2012
  • Status: Active Grant
First Claim
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1. a kind of preparation method of chicken necrotizing enterocolitis A type inactivated vaccine is it is characterised in that by A type bacillus perfringens CVCC37Strain seed liquor is inoculated in the liquid with pancreas casein peptone and yeast extract powder as main raw material(s) by the 1%~

  • 2% of culture medium total amountIn body synthetic medium, the culture/of 37 DEG C of anaerobic fermentations or quiescent culture 6 hours, culture terminate after through 3000r/min centrifugation30min, removes thalline, system is concentrated by ultrafiltration with the millipore of 8Ku molecular cut off respectively and is concentrated by ultrafiltration, through 0.6%Formalin inactivation detoxification complete after, add final concentration 20% aluminium hydroxide gel be configured to vaccine;

    Used in it, the formula (W/V) of synthetic medium is:

    Peptone 1.5g, yeast powder 0.5g, sodium chloride 0.25g, phosphoric acidHydrogen dipotassium 0.45g, glucose 0.5g, iron chloride 0.0001g, cysteine hydrochloride 0.05g, Vc0.001g, deionized water addsTo 100ml.

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