Cell culture in chitosan Alginate hydrogel globule

Cell culture in chitosan Alginate hydrogel globule

  • CN 102,753,205 B
  • Filed: 02/11/2011
  • Issued: 02/10/2016
  • Est. Priority Date: 02/25/2010
  • Status: Active Grant
First Claim
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1. production comprises a method for the hydrogel matrix of chondroblast, and described method comprises the steps:

  • -alginate soln is provided,-provide Mw lower than the chitosan soln of 60kD,-described alginate soln and described chitosan soln are mixed with chondroblast, wherein mixed solution comprises the chitosan of 0.5-0.7%w/v and the alginate of 1-1.4%w/v,-drop of described mixed solution is incorporated into there is Ca 2+or Sr 2+in cationic solution, and -from described, there is Ca 2+or Sr 2+the globule of cationic solution separating polymerization.

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