The B cell epitope peptide fragment of human neutrophil gelatinase-associated lipocalin and its application

The B cell epitope peptide fragment of human neutrophil gelatinase-associated lipocalin and its application

  • CN 102,775,473 B
  • Filed: 07/30/2012
  • Issued: 10/12/2018
  • Est. Priority Date: 07/30/2012
  • Status: Active Grant
First Claim
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1. the B cell epitope peptide fragment of human neutrophil gelatinase-associated lipocalin NGAL, it is characterised in that:

  • The peopleThe B cell epitope peptide fragment of neutrophil gelatinase-associated lipocalin has 18 amino acid, and amino acid sequence is such asSEQ ID NO;

    Shown in 3, wherein the B cell epitope peptide fragment obtains in the following manner:

    A) Chou&

    Fasman predictions β

    -bend, Emini methods prediction antigenic surface accessibility, Karplus&

    Schulz methods is utilized to predictAlbumen flexibility, Kolaskar&

    Tongaonkar proteantigens are analyzed, Parker method protein hydrophobics are analyzed,Bepipred linear epitopes are predicted and glycosylate information by obtaining NGAL in UniProt databases, and B cell is filtered outThe peptide fragment that epitopic features are apparent, immunogenicity is strong;

    B) amino acid number is selected again in apparent, the strong amino acid of immunogenicity peptide fragment from the B cell epitopic features that are filtered outThe target peptide fragment that amount is no less than 15;

    C) monoclonal antibody is prepared respectively using the target peptide fragment, select potency and the optimal monoclonal antibody of affinity,To obtain peptide fragment corresponding with selected monoclonal antibody, i.e., the described B cell epitope peptide fragment;

    The B cell epitope peptide fragment is used to prepare monoclonal antibody.

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