Method for culturing adherent cells

Method for culturing adherent cells

  • CN 102,782,121 A
  • Filed: 12/21/2010
  • Published: 11/14/2012
  • Est. Priority Date: 12/23/2009
  • Status: Active Application
First Claim
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1. method that is used to produce attached cell, according to this method:

  • A. attached cell is introduced and cultivated in the vessel, said cultivation vessel contain the microcarrier in substratum;

    B. successive cell goes down to posterity amplifying cells through in these identical cultivation vessel, carrying out for several times, wherein carries out the each passage behind first time passage;

    I. said cell colony is being implemented enzymically treat so that after said cell is broken away from from microcarrier, all or part cell colony that obtains during the passage before the priority of use;

    WithIi. through introducing the microcarrier of substratum and increasing amount;

    WithC. said cell colony is implemented enzymically treat so that after cell is broken away from from microcarrier, the cell colony that results are produced optional during the last passage.

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